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| ID | Components | Size |
|---|---|---|
| EP132-C01 | High-bind Plate | 1 plate |
| EP132-C02 | Human IL-4 R alpha | 30 μg |
| EP132-C03 | Human IL-4 | 20 μg |
| EP132-C04 | Human IL-4-Biotin | 10 μg |
| EP132-C05 | Streptavidin-HRP | 10 μg |
| EP132-C06 | Coating Buffer | 12 mL |
| EP132-C07 | 10xWashing Buffer | 50 mL |
| EP132-C08 | Blocking Buffer | 50 mL |
| EP132-C09 | Substrate Solution | 12 mL |
| EP132-C10 | Stop Solution | 7 mL |
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-4 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-4-Biotin to bind the coated human IL-4 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit IL-4: IL-4 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.
![IL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA KitIL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA Kit (Cat. No. EP-132) ELISA bioactivity](/static/main/products/images/elisa/EP-132-E1.jpg)
INHIBITION OF IL-4 [BIOTINYLATED]: IL-4 R ALPHA BINDING BY Human IL-4
Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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