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| ID | Components | Size |
| CRS014-C01 | Pre-coated Human CD28 Microplate | 1 plate |
| CRS014-C02 | Anti-CD28 Antibody Standard | 20 μg |
| CRS014-C03 | HRP-Goat anti-Mouse IgG | 10 μg |
| CRS014-C04 | 10xWashing Buffer | 50 mL |
| CRS014-C05 | 2xDilution Buffer | 50 mL |
| CRS014-C06 | Substrate Solution | 12 mL |
| CRS014-C07 | Stop Solution | 7 mL |
The kit is developed for the detection of anti-CD28 antibody in Bioprocess manufacturing applications. It is used as a tool to aid in routine quality control of in-process streams as well as final product.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) All reagents were returned to room temperature(20℃-25℃) before use.
b) Add your sample to the plate, take the Anti-CD28 Antibody as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add a diluted HRP-Goat anti-Mouse IgG to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of Antibody bound.
Detection of Monoclonal Anti-CD28 Antibody titer by indirect-ELISA Assay.
Immobilized Human CD28 can bind Anti-CD28 Antibody. Detection was performed using HRP-Goat anti-Mouse IgG with sensitivity of 0.16 ng/mL (QC tested).
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