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| Assay Type | Indirect-ELISA |
| Analyte | Anti-CD28 Antibody |
| Format | 96T(8×12 strips) |
| Reactivity | / |
| Regulatory Status | RUO |
| Sensitivity | ≤0.078ng/mL |
| Standard Curve Range | 0.078 ng/mL-2.5 ng/mL |
| Assay Time | 2 hr 20 min |
| Suitable Sample Type | For the detection of anti-CD28 antibody in Bioprocess manufacturing applications |
| Sample volume | 100 uL |
| ID | Components | Size |
| CRS014-C01 | Pre-coated Human CD28 Microplate | 1 plate(8×12 strips) |
| CRS014-C02 | Anti-CD28 Antibody Standard | 20 μg |
| CRS014-C03 | HRP-Goat anti-Mouse IgG | 10 μg |
| CRS014-C04 | 10xWashing Buffer | 50 mL |
| CRS014-C05 | 2xDilution Buffer | 50 mL |
| CRS014-C06 | Substrate Solution | 12 mL |
| CRS014-C07 | Stop Solution | 7 mL |
Detection of Monoclonal Anti-CD28 Antibody titer by indirect-ELISA Assay.
Immobilized Human CD28 can bind Anti-CD28 Antibody. Detection was performed using HRP-Goat anti-Mouse IgG with sensitivity of 0.16 ng/mL (QC tested).
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